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KMID : 1123920050190061636
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Korean Journal of Oriental Physiology and Pathology
2005 Volume.19 No. 6 p.1636 ~ p.1639
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In vitro Growth Inhibition and Apoptotic Effects of Hang-baek-Tang on HL-60 Cells
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Park Jun-Ho
Ju Sung-Min
Kim Kun-Jung
Jeon Byung-Hoon
Oh Jung-Mi
Lee Chai-Ho
Han Dong-Min
Kim Won-Sin
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Abstract
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To develop novel anti-leukemic medicine, we have prepared a Korean traditional medicine, named Hang-baek-Tang, which is composed of 8 kinds of anti-leukemic medicinal plants. The water extracts was examined anti-leukemic activity using the human leukemia cell line, HL-60 cells. HL-60 cells showed the growth inhibition and several apoptotic features, including DNA ladders, morphological changes, by treatment of the cells with Hang-Daek-Tang. We have observed that Hang-baek-Tang induced the activation of caspase-3, caspase-8 and caspase-9. Further molecular analysis demonstrated that Hang-baek-Tang induced cleavage of PARP and increase of hypodiploid (Sub-G1) population in flow cytometric analysis. These results indicate that Hang-baek-Tang has been considered to exert anti-leukemic activity through the procaspase-3 activation pathway.
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KEYWORD
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Apoptosis, caspase-3, PARP, HL-60 cells, leukemia
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